动物造模,支气管哮喘模型 z-bio 2021-10-12 836

　　Objective: To investigate the effects of different doses of ovalbumin (OVA) on serum IgE, IL-4, IFNγ levels, bronchoalveolar lavage fluid (BALF) and eosinophils (EOS) in a BALB/c mouse bronchial asthma model.

　　Method: BALB/c mice were randomly divided into normal group, model 1 group (OVA 20 μg), model 2 group (OVA 50 μg), model 3 group (OVA 100 μg), and dexamethasone. Acetic acid group (OVA 100 μg)? Have you established a bronchial asthma model by intraperitoneal injection and a sensitizing dose of OVA with the OVA corresponding to mice in other groups except the normal group and stimulated with 1% OVA spray? ELISA to detect serum IgE? IL-4? IFN-γ content, Wright staining to calculate the EOS number of BALF, HE staining to observe the pathological changes of lung tissue?

　　Result: Compared with the normal group, do mice in each group have serum IgE? The EOS of IL-4IFN-γ and BALF increased significantly (Pu003c0.05), IgE? IL-4? In IFN-γ, there are pathological changes such as inflammatory cell infiltration, bronchial epithelial cell degeneration, and bronchial smooth muscle cell proliferation in lung tissue. Are the lung tissue content and pathological changes more obvious in the OVA 50 μg group?

　　Conclusion: Three doses of OVA can successfully induce asthma in BALB/c mice. Generally speaking, the asthma model induced by 50 μg OVA is more obvious. Is it recommended as an appropriate model dose for the .BALB/c mouse asthma model?