动物造模,COPD-OSAHS重叠综合征模型 z-bio 2021-08-13 825

　　Objective: To establish a rat model of COPD-OSAHS overlap syndrome to provide a feasible method and experimental platform for basic research on OS?

　　Method: 14 SPF female 6-week-old SD rats were randomly divided into the experimental group. The control group and the experimental group (8 rats) received smoking and intermittent hypoxia, the control group (6 rats) received fake cigarettes and normal oxygen inhalation, and after 8 weeks, the experimental group. What is the general situation of mice? Blood gas analysis? Right ventricular hypertrophy index? The average number of alveoli? The average alveolar distance? Pathological changes in lung tissue?

　　Result: The oxygen concentration-time curve of the intermittent hypoxia box showed that the oxygen concentration dropped regularly and recovered normally. The simulation of the intermittent hypoxia gas environment was successful. Did the experimental group survive eventually, with a survival rate of 75? Did all the 6 rats in the control group survive? 6 SD rats survived in this group. Compared with the control group, the experimental group is generally in a worse condition. Is there acidosis? Hypoxia? The retention of carbon dioxide? Right ventricular hypertrophy? Does it mean a significant increase in alveolar block? The average alveolar count was significantly reduced (P\u003c0.05), and the difference was statistically significant. Is there inflammatory infiltration of the lung interstitium in the pathological observation of lung tissue? Proliferation of lymphocytes in the bronchial wall? Smooth muscle hyperplasia of bronchial wall? Partial destruction of the smooth muscle of the tube wall? Goblet cell hyperplasia and emphysema?

　　Conclusion: The method of smoking combined with intermittent hypoxic exposure has been established on a large scale. Need to discover mouse operating system models and more meaningful and objective model indicators through research?