动物造模,糖尿病大鼠模型 z-bio 2021-08-19 327

　　Objective: To observe the changes in the structure and abundance of the intestinal flora in a diabetic rat model induced by streptozotocin.

　　Method: 25 male SD rats were randomly divided into control group (C) 10 rats and model group (M) 15 rats. The model group was intraperitoneally injected with 3% STZ at a dose of 30 mg/(kg·bw) for 5 consecutive days. After forming the model, measure the weight, blood sugar and other indicators every month. When the model was established for 4 weeks and 12 weeks, fresh rectal feces from each group of rats were taken at the same time. The 16S rDNA-V3 region of bacteria in fecal samples was sequenced on the Illumina high-throughput sequencing platform, and the structure and abundance of the intestinal flora were quantitatively analyzed.

　　Result: There is no significant difference in the number of optimized sequences between the model group and the control group (P>0.05). The relative abundance index (Chao1) and diversity index Shannon of the intestinal flora of the model group were lower than those of the control group (P<0.05), and the Simpson index was higher than that of the control group (P<0.05). At the phylum level, the relative abundance of Proteobacteria, Cyanobacteria, Tenericutes, TM7, Actinobacter (Actinobacter) decreased (0 5). At the genus level, the relative abundance of Lactobacillus (Lactobacillus) in the model group decreased (P <0.05); the relative abundance of Bacteroides (Bacteroides) increased (P <0.05). After 12 weeks of modeling, the relative abundance of Lactobacillus (Lactobacillus), Bacteroides (Bacteroides), and Ruminococcus (Ruminococcus) in the model group increased (P <0.05); the relative abundance of Bifidobacteria (Bifidobacter) Decrease (P <0.05).

　　Conclusion: The abundance and diversity of the intestinal flora of diabetic animals in SD rats induced by STZ decreased, which provides a reference for the study of the relationship between diabetes and intestinal flora.