动物造模,小鼠化疗性肠黏膜炎模型 z-bio 2021-09-24 569

　　Purpose: To study 5-fluorouracil (5-fluorouracil, 5-FU) induced chemotherapy animal model of intestinal mucositis in mice.

　　Methods: Different doses of 5-FU were administered intraperitoneally or continuously for 5 days to mice. The body weight and diarrhea of the mice were observed daily. At 72 hours or 24 hours after the last administration, the peripheral blood and blood conditions of the mice were observed. Histopathological changes in the small intestine.

　　Results: Compared with the normal group, after a single or continuous 5-day administration of 5-FU, mice in each dose group experienced varying degrees of diarrhea and weight loss, and the levels of white blood cells and platelets in the peripheral blood were significantly reduced (P<0.05 or P < 0.01), the single-dose 400 mg/kg group and the continuous 50,100 mg/kg group showed obvious pathological features of intestinal mucositis. The 100 mg/kg group was overdose and the mortality rate reached 100%.

　　Conclusion: The effect of single or continuous 5-day administration of 5-FU on intestinal mucositis in mice is dose-dependent. 400 mg/kg is the appropriate dose for a single administration, and 50 mg/kg is the appropriate dose for 5-day administration. The right dose.