动物造模,牙周炎 z-bo 2022-02-04 461

　　(1) Replication method The periodontitis animal model was established by oral inoculation with Porphyromonas gingivalis. Adult rats weighing 250g were injected intramuscularly with kanamycin or ampicillin at a dose of 20mg/head 4 days before the experiment. 1 time/d to inhibit the endogenous bacteria that are not conducive to the colonization and growth of Porphyria gingivalis; after antibiotics are given for 3 days, take 50g/L of low-viscosity carboxymethylcellulose Porphyria gingivalis 381 suspension, Apply the bacteria to the gingival margin and even the entire oral cavity by tube feeding, once a day, for a total of 3 days.

　　(2) Model characteristics After 6 weeks of tube feeding Porphyria gingivalis suspension, it was found that the alveolar bone of the model rats was significantly lost, especially at the position of the adjacent surface of the molars, indicating that the single Porphyria gingivalis oral inoculation used in this method can induce The occurrence of periodontitis in model animals. Compared with the periodontitis animal model established by other methods, the method for making the model is simple and inexpensive.

　　(3) Comparative medicine. Clinically, local irritation such as plaque is the main pathogenic factor of periodontitis. However, some periodontitis, such as rapidly progressing periodontitis and juvenile periodontitis, are difficult to explain by local pathogenic factors alone. In the process of these periodontitis, systemic factors of the patient's body also play an important role. Some research reports have been confirmed by animal experiments that although oral vaccination with a single pathogen can cause experimental periodontitis in animals, the synergistic effect of multiple pathogens can significantly aggravate the development process and disease manifestation of periodontitis in model animals .