动物造模,咳嗽变异性哮喘模型 z-bio 2021-08-19 594

　　Objective: To sensitize Brown-Norway (BN) rats with ovalbumin (OVA) to establish a simple and easy animal model of cough variant asthma.

　　Method: 36 BN rats were divided into three groups: normal control group, model control group, and administration group. On the 1st day, the rats were injected intraperitoneally with 2mg ovalbumin and 100mg Al(OH)3 gel. Three weeks later, the rats were intraperitoneally injected again with 0.01mg OVA and 100mg Al(OH)3 gel. The rats in the normal control group were injected intraperitoneally with the same amount. Normal saline. After 3 weeks, the model control group and the administration group were nebulized with 1% OVA, and the normal control group was nebulized with normal saline, once every other day, for a total of 7 times. On the day of nebulization, the administration group was given intragastric administration, and the other two groups were given the same amount of normal saline once a day for a total of 14 times. Twenty-four hours after the last administration, capsaicin cough and lung function tests (provocation test) were performed.

　　Results: Compared with the normal control group, the number of coughs in the model control group increased significantly (P<0.01), the total inhalation airway resistance (RL) increased significantly (P <0.05), and the dynamic compliance (Cdyn) decreased significantly (P <0.05); Compared with the model control group, the number of coughs in the administration group was significantly reduced (P <0.05), RL was significantly decreased (P <0.05), and Cdyn was significantly increased (P <0.05) ).

　　Conclusion: This animal model is similar to many clinical features of cough variant asthma, and the operation is simple and easy, so it can be used as an animal model of cough variant asthma.