动物造模,高动力型感染性休克犬模型 z-bio 2021-08-20 552

　　Objective: To prepare a dog model of highly dynamic septic shock by intravenous injection of Escherichia coli.

　　Method: 22 mongrel dogs were randomly divided into control group (n=11) and experimental group (n=11). After anesthetizing the dog, a double-cavity central venous catheter was inserted through the right femoral vein. The control group used a micro pump to pump normal saline into the dog’s femoral vein. The experimental group was pumped with Escherichia coli in the same way, and the PICCO catheter was inserted through the right femoral artery. PICCO was used to monitor systemic hemodynamics at each time point between 0 and 48 hours.

　　Results: The heart rate (HR), cardiac output (CO), and stroke volume (SV) of the experimental group were significantly increased at 12 h (P<0.05), and the systolic blood pressure (SBP) and peripheral vascular resistance (SVR) were at 12 h. h was significantly reduced (P <0.05), while="" other="" hemodynamic="" indicators="" such="" as="" central="" venous="" pressure="" pulmonary="" artery="" wedge="" oxygen="" delivery="" consumption="" uptake="" rate="" were="" different="" none="" significant="" p="">0.05). A significant decrease in urine output can be observed in the experimental group from 12 h; peak blood flow velocity (PSV) and resistance index (RI) during arterial systole are significantly different from those in the control group (P<0.05).

　　Conclusion: The use of micro-pump to pump live E. coli into the canine femoral vein can successfully prepare a high dynamic septic shock dog model, which can provide an experimental basis for the study of septic shock.