动物造模,肠系膜上静脉血栓形成大鼠模型 z-bio 2021-08-20 523

　　Objective: To establish a rat model of superior mesenteric vein thrombosis by ligation, to simulate the pathological process of the disease, and to provide basic preparations for the study of its pathogenesis and treatment methods.

　　Method: 96 male SD rats were randomly divided into three groups, group A (sham operation group), group B (strangulation group) and group C (simple group), with 32 rats in each group. The rats in group A only opened the abdominal cavity and did not block the blood supply. The rats were sacrificed in batches at 8, 24, 48, 72 h after the operation; the rats in group B and C were ligated to establish the strangulated superior mesenteric vein thrombosis. Rats were sacrificed in batches at 8, 24, 48, and 72 h after modeling for the simple and simple model. HE staining was used to observe the morphological changes of the rat’s intestinal tissues and score the damage; ELISA was used to detect the levels of rat serum midgut fatty acid binding protein (IFABP) and α-glutathione S transferase (α-GST).

　　Results: The results of HE staining and pathological scoring showed that compared with the rats in group A, the intestinal tissues of rats in group B and C had different degrees of blood stasis and damage, group B gradually aggravated, and group C gradually reduced, and The degree of blood stasis and injury is positively correlated with the extent of ligation. The results of serum ELISA method showed that compared with the rats in group A, the levels of IFABP and α-GST in the serum of rats in group B and C increased to different degrees (P<0.05), and the degree of increase was positive with the range of ligation. Related.

　　Conclusion: This study used ligation to successfully establish a rat model of superior mesenteric vein thrombosis, which is simple and easy to perform, and has a high surgical success rate, and can be used in related studies.