动物造模,小鼠间断性禁食模型 z-bio 2022-01-27 753

　　Purpose: To explore a stable and reliable alternative to traditional calorie restriction in mice model establishment and its effect on glucose homeostasis.

　　Method: 40 SPF 16-week-old C57BL/6J mice (half male and half male) were randomly divided into four groups according to gender and feeding method. The free diet group was given basal feed freely, and the intermittent fasting group had normal diet and fasting alternately for 24 hours each, and they were allowed to drink freely on fasting days. The body weight and blood glucose changes of the mice in each group were monitored, and the transperitoneal glucose tolerance test and the transperitoneal insulin tolerance test were performed before and 12 weeks after the modeling.

　　Result: After 12 weeks of intermittent fasting, the weight and fasting blood glucose of the mice did not change significantly. After intraperitoneal injection of glucose, the increase in blood glucose was reduced, and the proportion of blood glucose decrease after intraperitoneal injection of insulin increased. Compared with the control group, the area under the curve of the tolerance experiment was significantly reduced (P<0.05).

　　Conclusion: After intermittent fasting, mice have increased insulin sensitivity and improved glucose tolerance, and the modeling method is simple to operate, which can be an effective alternative to traditional calorie restriction models.