动物造模,小鼠肝癌模型 z-bio 2021-08-20 386

　　Objective: To establish a diethylnitrosamine-induced liver cancer model in mice and use two different formula feeds: SPF grade mouse and rat complete compound feed and AIN93-G formula synthetic feed separately, to explore the effect of different feeds on the construction of DEN Inducing the effects of mouse liver cancer models.

　　Method: 14-day-old male C57BL/6 mice were injected intraperitoneally with a one-time injection of DEN solution at a dose of 25 mg/kg body weight to establish a mouse liver cancer model. After weaning, the mice were divided into two groups, one group was fed with regular SPF-grade full price compound feed for rats and mice, and the other group was fed with AIN-93G formula synthetic feed. The model mice were sacrificed when they were 9 months old, and the liver tissues were taken and weighed, and the growth of liver cancer was observed and recorded.

　　Results: All mice in the complete diet group developed tumors. The body weight and liver weight of the mice in the AIN-93G group were significantly lower than those in the complete diet group. The incidence of liver cancer and the number and size of tumor nodules were also significantly lower than those in the complete diet. Group mice.

　　Conclusion: The model of liver cancer induced by DEN in mice fed with complete diet was successfully established, while the occurrence and development of liver cancer induced by mice fed with AIN-93G diet was inhibited, and the weight of mice decreased significantly, suggesting the establishment of dietary factors in animal disease models. Play a key role in the