动物造模,胃癌原位移植模型 z-bio 2021-08-23 208

　　Objective: To study the application effect of NIRF dye in the in vivo imaging of orthotopic transplantation model of gastric cancer.

　　Methods: The human gastric cancer cell line HepG2 labeled with luciferin was transplanted orthotopically into nude mice to establish a tumor model, and at the same time, a gastric ulcer model was induced; bioluminescence imaging and NIRF imaging were used for the above models to observe the effect of gastric cancer tissue on near-infrared fluorescent dyes. Absorption; explore the effects of hypoxia and anion transport peptide (OATP) on the absorption of NIRF dyes by gastric cancer tissues, and clarify the specificity of NIRF dyes to target and recognize tumor cells.

　　Result: NIRF signal and bioluminescence signal have a good correlation in in vivo imaging of orthotopic transplantation model of gastric cancer. Strong NIRF fluorescence signal can be obtained in the part of gastric cancer tissue, but no fluorescence signal can be detected in the part of gastric ulcer. Hypoxia can enhance the absorption of NIRF dye by gastric cancer cells, and the anion transit peptide-specific inhibitor sulphobromide sodium (BSP) can significantly reduce the absorption of NIRF dye by tumor cells.

　　Conclusion: Heptamethine cyanine near-infrared fluorescent dye can target and identify the orthotopic transplantation model of gastric cancer.