动物造模,高脂血症模型 z-bio 2021-08-23 365

　　Objective: To feed SD rats with a high-fat diet for 8 weeks to establish hyperlipidemia, to verify the feasibility of this modeling method, and to explore the changes in blood lipids of rats at different time points.

　　Method: 30 normal male SD rats of 8 weeks old, after 1 week of adaptive feeding, were randomly divided into control group, model 1 group, and model 2 groups according to their body weight, with 10 rats in each group. The control group was given ordinary feed, and the model group 2 was fed high-fat and high-cholesterol feed for 8 weeks. The diet and drinking water of each group were measured daily, and the litter was changed and weighed for 4 days. At the 4th, 6th, and 8th week, the animals were fasted overnight and blood was collected from the orbital venous plexus to determine the four levels of blood lipids in each group. The animals were sacrificed at the end of the 8th week, and the liver and aorta of each group were collected for HE staining.

　　Results: Compared with the control group, the average daily dietary intake of the model 1 group increased, but the difference was not statistically significant; the daily average dietary intake of the model 2 group decreased significantly, and the difference was statistically significant (P<0.01). Compared with the control group, the daily water consumption of the model group 2 groups was significantly reduced, and the difference was statistically significant (P<0.01). Compared with the control group, the liver/body mass index of the model group increased significantly, and the difference was statistically significant (P<0.01). Compared with the control group, the final body weight of the model group 2 increased, but only the model 1 group was statistically significant (P<0.05). In terms of blood lipids, compared with the control group, the TC level of the model group 2 in the 4 weekend and 6 weekend model group was significantly increased, and the difference was statistically significant (P<0.01 or P<0.05). The LDL-c level of the 8 weekend model group 1 increased significantly High, the level of HDL-c decreases, and the difference is statistically significant (P<0.05). The HE staining of the liver showed that the liver cells in the control group were arranged normally and stained evenly, while the liver cells in the model group 2 had a large area of fatty degeneration, and a few liver sinusoids were congested and inflammatory cells infiltrated. HE staining of the aorta showed that the aortic structure of the three groups was normal.

　　Conclusion: SD rats fed with high-fat diet to establish hyperlipidemia is established, the model created is hypercholesterolemia and severe fatty liver. At the same time, during the experiment of hyperlipidemia in SD rats fed with high-fat and high-cholesterol feed, the fluctuation of blood lipids showed a state of increased-internal adaptation-increased. In the process of modeling, how to overcome the problem of animal anorexia and internal regulation of cholesterol metabolism is the key to high-fat feed feeding.