动物造模 z-bio 2021-09-16 613

　　OBJECTIVE: To establish a nude mouse human-derived adenomyosis model through different modeling methods, to screen out an ideal modeling method, and to provide an ideal animal model for studying the pathogenesis of adenomyosis and related treatment options.

　　Method: 80 female nude mice were randomly divided into abdominal implantation group, subcutaneous implantation group, intraperitoneal injection group and subcutaneous injection group, with 20 mice in each group. Each model group used different modeling methods to establish a nude mouse human-derived adenomyosis model. The volume and histomorphology of the graft were observed 4 weeks after the operation.

　　Results: The modelling success rate of the abdominal implant group was 95%, the modelling success rate of the subcutaneous implant group was 45%, and the modelling rate of the intraperitoneal injection and subcutaneous injection was 0%.

　　Conclusion: The nude mouse model of adenomyosis established by the abdominal cavity implantation method has high modeling rate and good stability. It is an ideal human-derived adenomyosis animal model.