动物模型,氟斑牙 z-bo 2020-08-08 731

　　(1) Reproduction method Select female rats weighing 70-100g after weaning, and use double distilled water to prepare different concentrations of sodium fluoride solution for poisoning, and the volume of poisoning is calculated as 0.5ml/100g body weight. The animals were given sodium fluoride solution on an empty stomach at a dose of 50mg/kg body weight every day, and the animals were fed by intragastric administration for 1 hour after exposure to the poison. The model animals were bred routinely with free drinking water (the fluoride content of tap water is 0.62mg/L) And eating (the fluorine content in the feed is 0.84mg/kg body weight). According to the same dose and method, each animal was exposed to the poison for 6 days a week and observed for 2 months.

　　(2) Characteristics of the model When the model animals were given sodium fluoride solution on the 20th to 25th day, all the fluorosis (Fluorosed Teeth) occurred. Most of the brown horizontal stripes on the tooth surface disappeared, and the entire tooth surface was white chalk-like with marginal defects. The changes in enamel gloss, enamel staining and enamel defects were similar to the clinical manifestations of human dental fluorosis. The model making method is simple, low cost and good repeatability.

　　(3) Comparative medicine The method of making this model adopts high fluoride solution to animals, which is in line with the cause of human dental fluorosis under natural conditions, and the clinical manifestations and pathological changes of dental fluorosis in model rats are related to the gloss and staining of tooth enamel. , Defects and other aspects are very similar to humans, so this model is suitable for research on the pathogenesis of human dental fluorosis and its prevention and control. At the same time, the production process and results of this model show that the dose of fluorosis is closely related to the incidence of dental fluorosis and the formation time of dental fluorosis. The greater the dose of fluorosis, the shorter the time for dental fluorosis to form, and the incidence is also Higher.