动物造模,异位肢体复合组织移植模型 z-bio 2021-09-27 332

　　Objective: To establish a stable heterotopic limb compound tissue transplantation model in mice.

　　Method: 25 pairs of C57BL/6 mice were selected for transplantation of heterotopic limb compound tissues of the same strain. The hind limbs of the donor mouse were taken and moved upwards along the femoral artery and femoral vein from the lower edge of the inguinal ligament. All branch vessels except the femoral vein and femoral artery were cut off by electrocoagulation to the external iliac artery and external iliac vein, and the external iliac artery and iliac vein were cut off. After the external vein, traverse the femur and femoral muscle. The donor external iliac artery and external iliac vein were anastomosed end-to-side with the right common carotid artery and external jugular vein of the recipient mice, respectively. Continuously suture the donor skin edge to the recipient skin edge to fix the limb. Cut off the tibia distal joint of the donor limb and ligate the stump.

　　Results: The success rate of the operation was 76%, the total operation time was (87.1±10.1) min, the donor acquisition time was (23.8±4.9) min, and the vascular anastomosis time was (55.3±9.6) min.

　　Conclusion: The mouse heterotopic limb composite tissue transplantation has a higher success rate, is stable and reliable, and is a new ideal animal model for research on transplantation immunity.