动物造模,急性肾损伤 z-bio 2021-09-16 361

　　Objective To explore the protective effect of curcumin on acute kidney injury induced by pregnancy-induced hypertension in rats and explore its potential mechanism.

　　Method 32 pregnant Sprague-Dawley female rats were divided into four groups: normal group, curcumin group, pregnancy induced hypertension (PIH) group and PIH+curcumin group. Rats in the PIH group and PIH+curcumin group were injected with 26 mol/L of NG-nitro-L-arginine methyl ester every day to induce the PIH model for 4 days. The rats in the curcumin group and PIH+curcumin group were fed curcumin 204.8 mol/L (suspended in 2% carboxymethyl cellulose solution) for 7 days from the 18th day. A series of indicators were detected by hematoxylin-eosin staining, enzyme-linked immunosorbent assay and spectrophotometry to evaluate the pathological morphology, renal function, oxidative stress and inflammation of the kidney tissue. Immunohistochemistry and Western blotting were used to detect the expression and distribution of TGF-β1 and p-Smad3.

　　As a result, compared with PIH group, the renal function of PIH+ curcumin group rats was significantly improved (P<0.01), and renal tissue injury and oxidative stress also had significant recovery (P<0.01), wherein the renal tissue score was determined by (191. 23±18. 82) dropped to (108. 35±11. 24) (P<0.01). The renal TGF-β1 and p-Smad3 of rats in the PIH group were significantly increased, but significantly decreased after the application of curcumin, and the expression of TGF-β1 decreased from (356.25±40.13)% to (178.40±18.45). )% (P<0.01), while p-Smad3 / Smad3 decreased from (312.82±30.26)% to (143.05±15.31)% (P<0.01).

　　Conclusion Curcumin can protect acute kidney injury in rats with pregnancy-induced hypertension by regulating the TGF-β1 pathway.