动物造模,小鼠免疫功能 z-bio 2021-09-19 318

　　Objective To investigate the effect of α-mangostin on the immune function of cyclophosphamide-induced immunosuppression in mice, and to provide experimental evidence for subsequent mechanism research and the development of new drugs that increase immune function.

　　Method The mice were randomly divided into 5 groups, each with 10 animals: blank control group, immunosuppressive group, α-mangostin high-dose group, middle-dose group and low-dose group. In the first 7 days, except for the blank control group, the other 4 groups were given cyclophosphamide (CTX) to create an immunocompromised model; after 14 days, the blank control group continued to be given intragastric saline, and the immunosuppression group was given intragastric administration. In the stomach corn oil, the three dose groups were given different doses of α-mangostin. The mice were sacrificed 24 hours after the last administration, and the delayed-type allergy (DTH) test, serum hemolysin test (HC50), macrophage phagocytic function test (carbon clearance test) and immune organ thymus and spleen index test were performed. Determination of killer cell (NK cell) activity (MTT method), determination of peripheral blood white blood cell count, and splenic lymphocyte proliferation test to study the effect of α-mangostin on the immune function of mice.

　　Result The mouse immunosuppression model was successfully created. After intragastric administration of α-mangostin, the three dose groups can increase the thymus and spleen index, half hemolysis value, splenic lymphocyte proliferation rate, and NK cell activity of immunosuppressive mice to varying degrees. Among them, the high dose group, the total α-mangostin The gavage dose of glutinin is 100 mg/(kg In delayed-type allergy, there are significant differences between the high-dose group and the blank control group and the immunosuppressive group (P<0. p="">0.05); in the peripheral blood white blood cell count determination experiment, the high-dose group is compared with the immunosuppressive group. The immunosuppressive group had extremely significant differences (P<0. p="">0.05); in the carbon clearance experiment, there was no statistically significant difference between each dose group and the blank control group and the immunosuppressive group (P> 0. 05).

　　Conclusion Mangostin has a regulatory effect on the immune function of immunosuppressed mice, and its optimal dose for improving the immune function of immunosuppressed mice is more biased towards the high-dose group, which is dose-dependent within a certain range.