动物造模 z-bio 2021-09-03 360

　　Objective To evaluate the feasibility and safety of two different left atrial appendage occluders, a single-disc cylindrical type and a double-disc split type, to occlude the left atrial appendage in animals.

　　Methods Fourteen healthy experimental dogs used single-disc cylindrical or double-disc split left atrial appendage occluder via femoral vein to perform left atrial appendage occlusion. Intraoperative left atrial angiography was used to evaluate the effect of the occlusion; the experimental dogs who died unexpectedly were immediately Gross specimens were examined to analyze the cause of death. The surviving experimental dogs were sacrificed on the 45th, 80th, 110th, and 15th months after the operation. The surface tissue of the occluder was stained with HE and DAPI and CD31 immunofluorescence to evaluate the degree of endothelialization.

　　Results Six experimental dogs used a single-disc cylindrical occluder for left atrial appendage occlusion. Five experimental dogs died 30 min to 8 hours after surgery, and one experimental dog survived to 15 months and was sacrificed; gross heart dissection revealed four There were a large number of thrombi on the surface of the occluder in the experimental dogs. About 1/5 of the occluder surface of the surviving experimental dog was not covered by new tissue; 8 experimental dogs used the double-disc split occluder for left atrial appendage occlusion, 7 The experimental dog was completely occluded; new tissue completely covered the surface of the occluder 110 days after the operation, and HE and immunofluorescence staining showed intact intimal formation, and new blood vessels were visible.

　　Conclusion The incidence of acute thrombosis and immediate death rate are high after the single-disc cylindrical occluder is implanted in the left atrial appendage; the double-disc split-type left-care ear occluder is used to block the left atrial appendage device-related complications after low left atrial appendage occlusion. The 4-mesh cover plate is completely intact.