动物造模,人脐带间充质干细胞 z-bio 2021-09-10 706

　　Objective This study explored whether transplantation of human umbilical cord mesenchymal stem cells can improve learning, memory and cognitive decline by regulating the autophagy level of naturally aging rats.

　　Methods SD rats were reared in a barrier environment to the aging age (24 months old). Behavioral studies selected old rats with significant cognitive decline and divided them into cell transplantation group (H group) and normal saline group (C group). SD rats were 3 months old. The rats are the normal group (N group). In group H, 500 μL of hUCMSCs cell suspension was injected into the tail vein at a dose of 2×10* each. Group C and N were injected with sterile normal saline through the tail vein. The time is once a week for 4 consecutive weeks. Morris water maze, Y maze and new object recognition experiments were used to observe the changes in learning and memory ability; HE staining was used to observe the morphological changes of rat hippocampal neurons; and Westem Blot was used to detect the relative expression of hippocampal LC3II/I, Beclin1, and P62.

　　Results (1) Behavioral results showed that the learning and memory ability of group H and group N was significantly higher than that of group C. (2) In the HE staining, the nerve cells in the hippocampal CA1 and DG areas of the H and N groups are arranged more regularly, the cell structure is complete, and the nucleus is full. The number of neurons in the CA1 area of the C group is reduced, the cell arrangement is loose, and the nucleus shrinks, showing degenerative Lesions. (3) The content of P62 protein in group H and group N was significantly lower than that in group C, and Beclin1 and LC3Ⅱ/I were significantly higher than that in group C.

　　Conclusion Intravenous injection of human umbilical cord mesenchymal stem cells improves learning, memory and cognitive decline in naturally aging rats, which may be related to the recovery of autophagy levels after intervention.