动物造模,原位移植瘤 z-bio 2021-09-06 280

　　Objective To investigate the inhibitory effect of the ethyl acetate extract of Saxifraga sylvestris on mouse liver cancer cells and orthotopic xenograft tumors.

　　Methods In vitro experiments, H22 cells were treated with different concentrations of EST for 48h, AO/EB dual fluorescence staining, and laser confocal microscopy were used to observe cell morphology changes; nucleic acid electrophoresis and flow cytometry were used to detect the apoptosis of H22 cells. Establish a mouse orthotopic xenograft model of liver cancer and conduct in vivo experiments to compare the effects of EST low, medium and high dose groups on tumor inhibition rate, T lymphocyte proliferation ability and liver pathological changes in tumor-bearing mice.

　　Results After EST treatment, H22 cells showed apoptotic morphological changes, which were positively correlated with the concentration; nucleic acid electrophoresis showed a characteristic ladder of apoptosis; flow cytometry analysis showed that the apoptosis rate of the 500μg/mL treatment group reached 31%; in vivo experiments showed , The tumor inhibition rates of low, medium and high doses of EST were 33.0%, 46.7%, and 64.3%, respectively; significantly improved the thymus index and T lymphocyte proliferation ability of mice (P<0.05); histological observation proved that each dose group of EST Cancer cells have different degrees of growth inhibition, pyknosis in morphology, and reduced infiltration into surrounding tissues.

　　Conclusion EST can enhance the immune function of mice and induce tumor cell apoptosis, and has obvious anti-tumor effects, which is positively related to the dose.