动物造模 z-bio 2021-09-06 803

　　Objective In the cultivation of sterile rats, the artificially formulated milk fed after birth is very important for the cultivation of sterile rats. Deeply hydrolyzed protein lactose-free milk powder is easy for infants and young children to absorb, reducing various indigestion and allergic reactions. Then adding this milk powder to the milk used by sterile rats will have a positive effect on the cultivation of sterile rats , And detect the biochemical indicators of successfully reared sterile rats.

　　Method Use clean SD rats to determine the labor period. After the uterus is removed, the rat pups are peeled off in an isolator, and they are artificially breast-fed until 22 days of weaning, and the body weight and survival rate are recorded. Among the formula milk used, the experimental group was added with deep-hydrolyzed protein lactose-free milk powder, and the control group was added with full-price formula milk powder. The blood biochemical indexes of the successfully cultivated sterile rats were tested at the 8th week.

　　Results In the first 14 days of artificial feeding, there was no difference in body weight and survival rate between the experimental group and the control group. From the 14th day, the weight and survival rate of the experimental group began to be higher than that of the control group, until the survival of the experimental group on the 22nd day. Compared with the control group, the rate was significantly increased (37.18% vs 17.78%), and the body weight on day 22 was also significantly higher than that of the control group (9.96±0.49) vs (13.36±0.59). The blood biochemical indexes of sterile rats showed a decrease in AST (aspartate aminotransferase) and an increase in GLU (glucose).

　　Conclusion In the process of cultivating sterile rat pups, the lactose-free milk powder with deep hydrolysis of protein can be added as an important component of formula milk, which can effectively increase the weight of pups and reduce mortality. The blood biochemical indexes of female sterile rats changed greatly.