动物造模,特异性心肌缺血 z-bio 2021-09-06 497

　　Objective To observe the expression of HE staining, VEGF (vascular endothelial growth factor) and FCF (fibroblast growth factor) in myocardial tissue slices caused by subcutaneous injection of isoproterenol for different days (1 mg/kg body weight) induced specific cardiomyopathy in rats And changes in the T wave of the ECG.

　　Methods Except for the blank group, rats in each group were injected subcutaneously with multiple points of isoproterenol (1mg/kg), respectively for the 2d group of continuous subcutaneous multiple points of isoproterenol (1mg/kg), and continuous subcutaneous multiple points of isoproterenol injection. In the 4d group (1mg/kg), continuous subcutaneous injection of isoproterenol (1mg/kg) for 6d group. One week after the administration, the rats were anesthetized, the electrocardiogram was measured, and the heart was taken. HE staining was used to observe the changes in the degree of injury of rat myocardial cells, and immunohistochemical staining was used to observe VEGF (vascular endothelial growth factor) and FGF (saturation Fibroblast growth factor) expression changes.

　　Results Compared with the blank control group, there were significant differences in the HE stained tissue slices under the microscope in the 6-d continuous subcutaneous injection of isoproterenol (1 mg/kg). At the same time, the model group’s myocardial tissue slices VEGF (vascular There are also significant differences in the expression of endothelial growth factor and FCF (fibroblast growth factor) compared with the blank control group.

　　Conclusion The results of this study show that compared with the blank control group, continuous subcutaneous injection of isoproterenol (1 mg/kg) for 6 days in the model rats caused by specific cardiomyopathy in myocardial tissue sections HE staining and VECF (vascular endothelial growth factor) , FGF (fibroblast growth factor) expression and changes in ECG T waves are significantly different. Therefore, rats with continuous subcutaneous multi-point injection of isoproterenol (1 mg/kg) for 6 days can be used as an experiment to evaluate the efficacy of specific cardiomyopathy. Use of animal models.