动物造模,载脂蛋白E敲除小鼠 z-bio 2021-09-06 571

　　Objective To study the effects of western diet and standard diet on hematology and pathology of apolipoprotein E knockout mice (ApoE-/-).

　　Method 3 month-old ApoE-/- mice were fed with Western dietary feed and standard feed, and the peripheral blood and aortic arch of the mice were collected at the time of feeding for 3 months, 4 months and 5 months. The blood lipid level was detected by an automatic blood analyzer, and the formation of atherosclerotic plaque was observed by sectioning and staining.

　　Results In terms of blood lipids, the total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in serum of mice fed with western diet and standard feed were significantly higher than those in control C57 mice. Except for the HDL-C of mice fed for 4 months, the blood lipid index of the western diet fed group was significantly higher than that of the standard diet group at all months of age; in the pathological section and staining of the aortic arch, the western diet group The aortic plaques in mice were obvious and the incidence of AS was significantly increased.

　　Conclusion Western dietary diet and standard diet can induce ApoE-/- mice to show AS symptoms, and western dietary diet has a more obvious induction effect.