动物造模 z-bio 2021-10-07 343

　　Objective To study the toxicity of oral administration of different extracts of Psoralen to rats and provide a safety basis for its clinical application.

　　Methods 112 SD rats were half male and half male. They were randomly divided into 8 groups according to gender and body weight: control group, psoralen crude drug group, psoralen lower layer fluid high and low dose groups, and psoralen upper layer fluid The high and low dose groups, the high and low dose groups of psoralen percolation dregs (the crude drug group dose (crude drug quantity) is 3 g/kg body weight, and the high and low doses (crude drug quantity) of the other administration groups are 6 respectively. And 3g/kg body weight], each group of 14 animals. The drug was administered continuously for 4 weeks. After the period of administration, fasting and water for 12 hours, blood was collected for detection of relevant blood biochemical indicators, organs were dissected and weighed to calculate organ coefficients, and MDA in liver tissue was determined, and histopathological examinations.

　　Results Compared with the control group, the liver organ coefficient, ALP and MDA of the psoralen crude drug group were significantly increased, and the thymus organ coefficient and T-AOC were significantly reduced; -AOC and ALT were significantly increased; the liver organ coefficient and MDA of the high-dose psoralen osmotic drug group increased significantly, while the T-AOC value of female rats decreased significantly. The low-dose drug residue group had no obvious liver and kidney Toxicity: There was no significant difference in the upper exudate group of psoralen.

　　Conclusion The safety of the upper layer of psoralen is better than the lower layer of psoralen, but based on the results of the active ingredient determination, the lower layer of percolation is more suitable for human medicine.