动物造模,腹泻型肠易激综合征 z-bio 2021-09-07 243

　　Objective: To investigate the immunoregulatory effect of p38MAPK signal in diarrheal irritable bowel syndrome rats.

　　Methods: 40 SPF Wistar rats (male and female) were randomly divided into D-IBS model I (modeling 7d) group, II (modeling 14d), III (modeling 21d) group and blank control group. 10 per group. The model group was modeled by chronic restraint combined with senna gavage, and the blank control group was given the same volume of purified water. D-IBS rats were killed on the 7th day, 14th day and 21st day of modeling, and the blank control group was killed on the 21st day. Blood was collected from the heart, and the levels of serum IL-1β, IL-6 and TNF-α in each group of rats were detected by enzyme-linked immunosorbent assay (ELISA); the colon tissue was dissected, and HE staining was used to observe the morphology of rat colon tissue and immunohistochemistry Method to measure the protein expression of p38MAPK in rat colon tissue.

　　Results: Compared with the blank control group, the serum levels of IL-1β, IL-6 and TNF-α and the positive rate of colonic p38MAPK protein expression in the model group increased (P<0.05, P<0.01), and the expression of p38MAPK and IL- The levels of 1β, IL-6 and TNF-α are positively correlated.

　　Conclusion: The D-IBS rat model reconstructed by chronic restraint combined with senna gavage may activate the p38MAPK signaling pathway to up-regulate the expression of p38MAPK and promote the release of IL-1β, IL-6 and TNF-α. Induces low-grade inflammation of the intestinal mucosa.