动物造模 z-bio 2021-09-07 213

　　Objective: To explore the scheme of electrical stimulation of the penile cavernous nerve to detect intracavernous pressure, and to compare the difference between two catheterization materials (PE-50 tube needle and intravenous infusion needle) for detecting intracavernous pressure (ICP) .

　　Methods: 36 SPF male Sprague Dawley rats were selected, 16 were randomly selected as the control group, and the remaining 20 were the experimental group. They were injected intraperitoneally with streptozotocin 60 mg/kg. After 8 weeks of rearing, 16 type I rats were selected. Diabetic rats. The control group and the diabetes group were divided into the PE-50 tube needle and the intravenous infusion needle group to record 8 ICPs. Masson's trichrome staining and apomorphine experiment confirmed that the model of diabetic ED rat model was successfully established. After the successful establishment of the model was confirmed, the intracavernosal pressure of the penis was tested, and the related erectile function data recorded by the PE-50 tube needle and the intravenous infusion needle were compared.

　　Result: Masson's trichrome staining showed that the diabetic model group compared with the control group, the area of corpus cavernosum smooth muscle decreased, the area of collagen increased, the area of corpus cavernosum smooth muscle/collagen decreased, the difference was statistically significant (P<0.05). The number of erections in the diabetes model group was significantly less than that in the control group (P<0.01). the="" peak="" icp="" and="" mean="" arterial="" area="" under="" p="">0.05) recorded by the pe-50 tube needle and the intravenous infusion needle in the normal group and the diabetes group, and the peak rate (slope) is statistically significant (P<0.05 or P<0.01).

　　Conclusion: PE-50 tube needle and intravenous infusion needle are both suitable for measuring intracavernosal pressure when electrical stimulation of the penile cavernous nerves is used to record intracavernous pressure.