动物造模,骨丢失 z-bio 2021-09-07 308

　　Objective: To explore and compare the effects of simvastatin and risedronate sodium alone or in combination on dexamethasone-induced osteoporosis in mice.

　　Methods: Thirty 12-week-old female C57BL6 mice were randomly divided into 5 groups (6 in each group): control group (group A), model group (group B), simvastatin group (group C), risedronic acid Sodium group (D group) and combined intervention group (E group). Except for group A, mouse osteoporosis models were prepared by tail suspension. Groups C, D, and E were given simvastatin (5 mg/(kg·d) )) ､Risedronate sodium (1mg/(kg·d)) and the combined intervention of the two, and sacrificed after 3 weeks to obtain samples. Micro-computed tomography (micro-CT) detection of the cancellous bone and cortex of the proximal tibia on the left Bone mass and microstructure parameters, three-point bending experiment to analyze the biomechanical properties of the left femur. RNA from the right tibia was extracted, real-time fluorescent light quantitative polymerase chain reaction (PCR) was used to detect the expression of OPG and RNAKL, and the right femoral protein was extracted, Western blot method to detect the expression of OPG and RNAKL protein.

　　Results: Micro-CT: B group trabecular bone volume ratio (BV/TV) and bone trabecular number (Tb.N) were significantly lower than the other groups, and the bone trabecular separation (Tb.Sp) was significantly higher than the other groups. Group (P<0.05), BV/TV in group C and D was significantly lower than that in group A and E, and Tb.Sp was significantly higher than that in group A (P<0.05); ②Biomechanics: maximum load and elastic modulus in group B were significantly lower There was no statistical difference between the A and E groups (P<0.05) and="" the="" other="" groups="" p="">0.05); ③PCR detection results: OPG mRNA expression in the C and E group was significantly higher than that in the B group (P<0.05); The expression of RNAKL in group B was significantly higher than that in group A (P<0.05); ④Western blot: the expression of OPG in group A was significantly higher than that of the other groups (P<0.05), and the expression of OPG in group A was significantly higher than that in group B (P<0.05); The expression of RANKL in group A was significantly lower than the other groups (P<0.05).

　　Conclusion: Tail suspension can cause bone loss and bone quality decline in mice. The combined application of simvastatin and risedronate sodium can prevent bone loss and bone quality decline in this model, and the effect is better than that of single medication.