动物造模 z-bio 2021-09-07 438

　　Objective: To preliminarily explore the effect of high Axl expression on subcutaneous tumor formation of gastric cancer cells in nude mice.

　　Methods: The relationship between Axl and gastric cancer was analyzed by pathological staining of gastric cancer; gastric cancer cells with high Axl expression were constructed by transfection of lentivirus; gastric cancer cells were injected subcutaneously to establish a nude mouse gastric cancer cell subcutaneous tumor model; by small animal in vivo imaging To assess the size of the subcutaneous tumor in vivo.

　　Results: The immunohistochemical results of tissues removed from gastric cancer surgery patients showed that the expression of Axl in gastric cancer tissues was significantly increased; the expression of Axl in the constructed Axl high-expressing gastric cancer cells increased by 2.4 times. The use of Axl high-expressing gastric cancer cells to establish nude mice subcutaneously Tumor formation model, small animal live imaging showed that 2 weeks after subcutaneous injection of cells, the subcutaneous tumor formed by high-expressing Axl cells was significantly larger than that of the control group, and after 4 weeks, the difference was more significant (P<0.01). Finally, high-expressing Axl gastric cancer cells The volume and weight of the formed subcutaneous tumors were significantly larger than those of the control group.

　　Conclusion: High expression of Axl in gastric cancer tissue, high expression of Axl can enhance the subcutaneous tumor formation ability of gastric cancer cell SGC-7901.