动物造模 z-bio 2021-09-07 400

　　Objective: To preliminarily explore the effect of camel spines extract pretreatment on the survival rate of rats in dry heat environment.

　　Methods: 50 SPF male SD rats were randomly divided into 5 groups: blank group (NC group), solvent group (SC group), camel spur low-dose group (AL group), camel spur middle-dose group (AM group) ､Camel spiny high-dose group (AH group), 10 per group. SC group was given 0.5% sodium carboxymethyl cellulose solution by gavage, and the high, medium and low-dose groups of Camel spiny extract were given 1g. /kg､0.33g/kg､0.10g/kg. Rats in each group were given intragastric administration once a day, and placed in an experimental chamber in a dry heat environment after 7 consecutive days. Temperature: (41±0.5)℃, relative humidity: (10 ±1)%. Record rat core body temperature, body weight and time of death at 0, 50, 100, and 150 minutes respectively.

　　Results: There was no significant difference in the daily weight change of rats in each group during the continuous administration period (P>0.05). After exposure to dry heat for 50, 100, and 150 minutes, the core body temperature of the AM and AH group was significantly lower than that of the NC and SC group (P<0.05). there="" was="" no="" significant="" difference="" in="" the="" rate="" of="" weight="" loss="" between="" groups="" p="">0.05). The survival time of rats in the dry heat environment in the AM group [(242.15±12.72)min] and the AH group [(244.90±12.68)min] were higher than those in the NC Group [(225.90±10.32)min]､SC group[(228.22±7.59)min]､AL group[(227.90±12.25)min] significantly prolonged (P<0.05), the survival rate was compared with NC group by LogRank test. ､SC､AL group significantly increased (P<0.05).

　　Conclusion: The study found for the first time that the pretreatment of camel thorn extract may increase the heat tolerance of rats in dry heat environment by delaying the rise of core body temperature, thereby increasing the survival rate of rats in desert dry heat environment. The mechanism remains to be further studied.