动物造模,糖尿病肾病 z-bio 2021-09-07 427

　　Objective: To study the effect of quinsulfacyclohexanone on diabetic nephropathy in rats and its effect on the expression of renal angiotensin Ⅱ and vascular endothelial growth factor.

　　Methods: Select 40 healthy SD rats of 8 weeks old, 10 of them were randomly selected as the normal group, and the other 30 were established as rat models of diabetic nephropathy. After the model was successful, the two groups of diabetic nephropathy rats were randomly divided into model groups of 10 and 10 There were 20 rats in the administration group, and every 5 rats in the administration group were injected with the same dose, respectively, with 5, 10, 15 and 30 mg/kg of quinsulfone cyclohexanone. The rats in the control group and the model group were given the same dose of physiology. Gavage with saline. Test the urinary albumin excretion rate (Uaer) and glomerular filtration rate (GFR) of rats in the administration group and the normal group. For the three groups of rats, fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), and insulin Resistance index (HOMA-IR), 24h urinary microalbumin (24hU-mAlb), β2 microglobulin (β2-MG), blood urea nitrogen (BUN) levels are detected, and monocyte chemotaxis in rat kidney tissue Protein-1 (MCP-1) mRNA, lectin-like oxidized low-density lipoprotein receptor (LOX-1) mRNA, AngⅡ, and VEGF expression levels, and compared between groups. The results were rats at a dose of 15 mg/kg The Uaer､GFR level is close to the normal Uaer､GFR level, so 15mg/kg is considered as the appropriate dosage. The following studies are conducted at a dose of 15mg/kg. The model group and the administration group are FBG, HbA1c, HOMA- IR, 24hU-mAlb, β2-MG, and BUN levels were higher than those in the normal group (P<0.05); the expression levels of MCP-1mRNA, LOX-1mRNA, and AngⅡ and VEGF in the kidney tissue of the model group and the administration group All were higher than the normal group (P<0.05); FBG, HbA1c, HOMA-IR, 24hU-mAlb, β2-MG and BUN levels in the administration group were lower than those in the model group (P<0.05). The administration group rats The expression levels of MCP-1mRNA, LOX-1mRNA, AngⅡ, and VEGF in kidney tissues were lower than those in the model group, with statistical differences (P<0.05).

　　Conclusion: Quinsulfacyclohexanone may inhibit the expression of AngⅡ､VEGF in the kidney tissue of diabetic nephropathy rats, improve the renal function and kidney disease of the rats, and ultimately play a role in kidney protection.