动物造模,尿毒症 z-bio 2021-09-08 245

　　Objective: To compare the application of two hemostasis methods in rat uremia model.

　　Methods: Male SD rats were divided into two groups A and B. Both left kidney 2/3 resection and right nephrectomy were used to make uremia models. Group A used 3M tissue glue for hemostasis during left kidney 2/3 resection, and group B used electricity. Blood coagulation and hemostasis, intraoperative blood loss and left renal blood flow blocking time, postoperative comparison of rat survival rate, measurement of creatinine value, to reach 2 to 3 times the normal blood creatinine value is a rat model of uremia. Kidney tissue Perform pathological examinations.

　　Results: The blood creatinine of the two hemostasis methods increased as much as the requirements of the uremia model; the blood flow block time of the left kidney in group A was (59.97±7.56) s, group B was (174.1±15.28) s (P<0.05); A The bleeding volume of left nephrectomy in group was (0.11±0.037) mL, group B was (0.24±0.056) mL (P<0.05); the pathology of renal tissue in both groups showed glomerular ischemia shrinkage and tubulointerstitial fibers The postoperative survival rate of group A was significantly higher than that of group B (P<0.05).

　　Conclusion: 3M tissue glue is superior to electrocoagulation to stop bleeding in terms of bleeding time, bleeding volume, and postoperative survival rate.