动物造模 z-bio 2021-09-19 300

　　OBJECTIVE: To investigate the neurotoxic effects of flame retardant TCPP exposure on mice and the study of related mechanisms

　　Method: 30 adult KM mice were randomly divided into normal control group (0 mg / (kg·d)) ､ low dose (TCPP) group (10 mg / (kg·d))

　　And the high-dose TCPP group (100 mg / (kg·d)) continued to be administered intragastrically for 30 days. After the exposure, the body weight and general conditions were observed. The learning and memory ability of the mice was tested by the water maze test. The chemiluminescence immunoassay was used. Determination of mouse serum total triiodothyronine (TT3), free triiodothyronine (FT3), total tetraiodothyronine (TT4) and free tetraiodothyronine (FT4) ) The level of glutathione transferase (GST), superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) levels in mouse brain tissues was determined by colorimetry

　　Results: Compared with the control group, the water consumption of the TCPP high-dose group was significantly decreased (P<0 05), and the liver and spleen organ indexes were significantly increased (P<0 05) The TCPP-exposure group escaped in the water maze experiment The incubation period was longer than that of the normal group of mice (P<0.05) The total swimming distance of mice in the high-dose group was significantly increased (P<0.05) and the stay time in the target quadrant was also significantly shortened (P<0.05) High-dose TCPP exposure Compared with the control group, the TT3 and FT3 of the mice in the group were significantly increased (P<0.05) Compared with the control group, the GST and SOD of the mice in the high-dose TCPP group were significantly reduced, and the MDA was significantly increased (P<0.05) Compared with mice in the control group, the mice in the low-dose TCPP-exposed group only showed a decrease in GST and an increase in MDA (P<0.05)

　　Conclusion: TCPP exposure has obvious neurotoxic effects and can cause the loss of learning and memory in mice. Its toxic mechanism may be related to brain oxidative damage and increased thyroid hormones.