动物造模 z-bio 2021-09-19 345

　　Purpose: To wrap Nile Red in a nano-preparation, and use its fluorescence characteristics to investigate the tumor targeting and tissue distribution of the nano-preparation in tumor-bearing mice.

　　Methods: The CCK-8 method was used to detect the cytotoxicity of Nile Red Nanoemulsion [NRNE(O)] to H1688. The BALB/c nude mouse lung cancer model was established, and Nile Red Suspension (NRS) and NRNE(O) were administered intragastrically. ), observe the dynamic distribution of fluorescence intensity in tumor-bearing nude mice through the small animal in vivo imaging system.

　　Results: NRNE(O) has low cytotoxicity and good biocompatibility, and its absorption in the body and accumulation of tumor sites are higher than NRS; NR can be quickly, stably, and clearly reflected in nano-formulations The dynamic distribution of nano preparations in mice.

　　Conclusion: The NRNE(O) prepared in this study has good stability, which provides an important research method for the application of Nile Red as a tracer in the study of nanoformulations in animals.