动物造模 z-bio 2021-09-08 594

　　Objective: To detect the effect of high-fat food and K / BxN serum on the distribution of immune cells in ApoE knockout (ApoE- /-) mice

　　Method: ApoE- /-mice were fed high-fat foods from 8 weeks of age and injected intraperitoneally with anti-glucose-6-phosphateisomerase (GPI) antibody-positive K/BxN serum weekly at 17 weeks of age. One injection of 0 2 mL per injection. The control group was fed with ordinary food. The same method was used to inject K/BxN serum. ELISA method to detect the changes in the blood lipid level of mice before and after the injection of K/BxN serum. / Spleen cells and bone marrow cells were separated after BxN serum treatment to 26 weeks of age. After flow cytometry antibody staining, flow cytometry analysis of the distribution of immune cells

　　Results: ApoE- /-mice were fed with high-fat food before and after intraperitoneal injection of K / BxN serum. TG) concentrations were significantly increased, and the plaque area at the root of the aortic valve was significantly increased, suggesting that no matter whether K/BxN serum high-fat food is injected or not, ApoE- /-mice can develop arteriosclerosis symptoms and are better than ApoE- /-fed with ordinary food. -Mice were more severely intraperitoneally injected with K/BxN serum before each group’s ApoE- /-Mice’s ankle joints and arthritis scores (clinical score) were all at normal levels after intraperitoneal injection of K/BxN serum and fed with high-fat food ApoE- / -Mouse ankle joint width and arthritis scores are lower than those of ApoE-/- mice fed with normal foods. Flow cytometry results show that after feeding high-fat foods, K/BxN serum was injected to make ApoE-/- mice spleen and bone marrow CD3+ T cells and CD19+ B cells are down-regulated and CD11b+ macrophages are up-regulated

　　Conclusion: High-fat food and K/BxN serum caused the down-regulation of CD3+ T cells and CD19+ B cells in the spleen and bone marrow of ApoE- /-mice and the up-regulation of CD11b+ macrophages, which resulted in aggravation of arteriosclerosis symptoms and reduction of arthritis symptoms in ApoE- /-mice