动物造模 z-bio 2021-09-08 318

　　Purpose: MMP12, also known as macrophage elastase, can almost decompose various extracellular matrix components and vascular components and is related to monocyte recruitment. It is also found that MMP12 is involved in tumor cell invasion and metastasis. In addition, MMP12 is also closely related to adipose tissue. In exploring the changes of macrophages in the blood and white fat of MMP12 knockout (MMP12- /-) mice

　　Method: Use PCR to perform genotype identification of MMP12- /-mice. Use littermate MMP12+ / + male mice as a control to test the blood routine of the two groups of mice and analyze them. Further use flow cytometry to detect macrophage markers. Changes in macrophages Finally, immunohistochemistry and other methods were used to observe the changes in macrophages in adipose tissue after MMP12 deletion

　　Results: (1) Identify the genotype of MMP12- /-mice and expand the population (2) Compared with littermate MMP12+ / + male mice, the red blood cell count, hemoglobin amount, platelet count, mononucleus of MMP12- /-male mice The number of cells and their percentages decreased significantly (P<0 05), the percentage of lymphocytes and the percentage of eosinophils increased significantly (P<0 05), and there was no statistical difference in their absolute counts (3) Flow cytometry results suggest that: The ratio of CD11b, a specific marker of phages, to F4/80 double-positive cells decreased significantly in the blood, and its absolute count also decreased (4) The results of H&E staining and immunohistochemistry showed that: compared with littermate MMP12+ / + male mice The expression of CD68 in white adipose tissue of MMP12- /-male mice was significantly increased

　　Conclusion: It is suggested that the changes in macrophages may be related to the knockout of MMP12. The significance lies in the discovery that MMP12 may regulate the differentiation of macrophages. The decrease of macrophages in the blood increases the number of macrophages in white fat.