动物造模,慢性肾病小鼠模型肾纤维化 z-bio 2021-09-08 381

　　Objective: To study the protective effect of curcumin on renal fibrosis in chronic kidney disease, and to explore the possible mechanism of action.

　　Methods: Thirty C57BL/6J mice were randomly divided into sham operation control group (NC), 5/6 renal ligation model group (LIG) and curcumin treatment group (LIG+CUR), 10 in each group, according to the modified 5/ 6 Kidney ligation method was used to make CKD animal model. Mice in the curcumin group were given curcumin-containing feed (100 mg/kg per day), and the other groups were given normal feed. After three months, the mice were sacrificed to detect the fibrosis index α-SMA and participate in chronic disease. Yap, the transcriptional activator of the Hippo pathway in nephropathy fibrosis. Results The results of renal function test showed that compared with the sham operation group, the urea nitrogen (BUN) and blood creatinine (Scr) in the ligation group were significantly increased. Curcumin can effectively protect the kidneys. Impaired function; H&E, Masson and immunohistochemistry results showed that the kidneys of mice in the ligation group had obvious renal tubular lesions and a certain degree of fibrotic changes, and the degree of fibrosis was significantly reduced after curcumin was administered (P<0.05); and The mRNA and protein levels of Yap protein increased after modeling, but decreased significantly after curcumin treatment (P<0.05).

　　Conclusion: Curcumin can effectively improve chronic kidney disease induced by 5/6 kidney ligation and reduce fibrotic lesions. The preliminary findings of the mechanism suggest that it may be related to the reduction of Yap expression in the Hippo signaling pathway.