动物造模 z-bio 2021-09-08 460

　　Purpose: To observe the effect of coumarin on anti-depression, and to preliminarily explore its possible mechanism of action.

　　Methods: A mouse chronic unpredictable mild stress (CUMS) model was used to evaluate the anhedonia of mice treated with plumarin by using a sugar water preference experiment. The serum of mice was measured by enzyme-linked immunosorbent assay. The content of corticosterone, the LC-MS/MS method was used to detect the content of various neurotransmitters in the mouse cortex and hippocampus.

　　Results: Cajanusin can reverse the decrease in sugar water preference index and the increase in serum corticosterone levels caused by CUMS. And compared with the normal group, the content of related neurotransmitters in the cortex and hippocampus of the model group was significantly changed. The administration of xylanin has a significant regulatory effect on related neurotransmitters in CUMS mice.

　　Conclusion: Cajanusin may achieve anti-depressant effects by reducing serum corticosterone levels and regulating neurotransmitters in the brain.