动物造模,抑郁模型 z-bio 2021-09-09 186

　　Purpose: To explore the effect of fluoxetine on the behavior and hippocampal neurogenesis of post-stroke depression (PSD) model rats.

　　Methods: Thirty-two adult male rats were randomly divided into sham operation group, stroke group, PSD group and fluoxetine group. The middle cerebral artery embolization method was used to prepare the ischemic stroke rat model, chronic unpredictable mild stimulation combined with orphaning method Preparation of PSD rat model. Body weight was measured at baseline and on the 7th, 14th, 21st, 28th and 35th day after surgery, as well as the sucrose preference test and open-box test to evaluate the behavioral changes of rats, and the forced swimming test was added on the 35th day ｡ At the end of the experiment, immunohistochemistry was used to detect the new generation of neurons in the hippocampal dentate gyrus.

　　Results: At 28 and 35 days after the onset of stress, the weight of rats, the proportion of sucrose water consumption, the horizontal and vertical movement scores of the open-box test, the PSD group was significantly different from the stroke group (P<0.05), fluoxetine The difference between the PSD group and the PSD group was also statistically significant (P<0.05). Compared with the stroke group, the immobility time of rats in the PSD group was significantly increased in the forced swimming test, and the swimming and climbing time was significantly shortened (P<0.01). The immobility time of rats after cetine intervention was significantly shorter than that of the PSD group, and the swimming time was significantly increased (P<0.01). Immunohistochemistry showed that compared with the stroke group, the number of new neurons in the hippocampal dentate gyrus of the PSD group was significantly reduced (P<0.01), and the number of newborn neurons in the hippocampal dentate gyrus of rats after fluoxetine intervention was significantly higher than that in the PSD group (P<0.01).

　　Conclusion: Fluoxetine can effectively improve the depression of PSD rats, and the regeneration of hippocampal neurons participates in this process and plays an important role.