动物造模 z-bio 2021-09-10 327

　　Objective: To explore the interventional effect and mechanism of low molecular weight heparin calcium on mouse embryo implantation.

　　Methods: Construct a mouse pregnancy model, intervene with LMWHC intraperitoneal injection, observe the effect of LMWHC on mouse embryo implantation. Isolate and culture mouse trophoblast cells, intervene with different concentrations of LMWHC cells, use Transwell chamber to detect cell invasiveness, use ELISA. Western blot and RT-PCR methods were used to detect the protein and mRNA levels of matrix metalloproteinases-MMP-2 and tissue inhibitor of metalloproteinase-TIMP-2 in the cell supernatant.

　　Results: After intraperitoneal injection of LMWHC in pregnant mice, the number of embryo implantation in D6 and D9 mice was significantly increased (P<0.05); 1×102 IU/L, 1×103 IU/L and 1×104 IU/L were used After 24h of LMWHC intervention with trophoblast cells, the number of trophoblast cells invaded was (84.83±14.15), (162.48±25.49), (98.73±12.77), and (65.27±13.92) without LMWHC intervention. ) Comparison, the difference was significant (P<0.05), the expression level of MMP-2 in the trophoblast cell supernatant was higher than that in the cells without LMWHC intervention (P<0.05), and the TIMP in the trophoblast cell supernatant -2 expression level is lower than that of cells without LMWHC intervention (P<0.05).

　　Conclusion: LMWHC can promote mouse embryo implantation, and its mechanism may be related to the enhancement of trophoblast cell invasion by LMWHC by affecting the expression of MMP-2 and TIMP-2.