动物造模,大鼠心肌氧化应激 z-bio 2021-09-10 535

　　Objective: To observe the effect of curcumin pretreatment on myocardial oxidative stress and cell apoptosis in rats with heat stroke in dry heat environment and to explore its possible mechanism.

　　Methods: Fifty male SD rats were randomly divided into 5 groups (n=10): normal temperature control group (NC), dry heat control group (DHC), curcumin 50mg/kg pretreatment group (L-cur), curcumin 100mg/kg (M-cur), curcumin 200mg/kg (H-cur). The NC and DHC group were given normal saline by gavage, and the curcumin pretreatment group was given different concentrations of curcumin by gavage, once a day for 7 consecutive days. Days. On the 8th day, except for the NC group, the other 4 groups of rats were transferred to the northwest special environment artificial experimental cabin for the experiment. The ambient temperature was (41 ± 0.5) ℃, and the humidity was (10 ± 1)%. It was reached at the 150th minute of the experiment Heat stroke state, take the material after anesthesia, use an automatic biochemical detector to detect the changes of serum creatine kinase (CK) and lactate dehydrogenase (LDH) in rats, use TUNEL method to detect cardiomyocyte apoptosis and calculate cardiomyocyte apoptosis Rate. Detect the content of myocardial superoxide dismutase (SOD) and malondialdehyde (MAD), and perform correlation analysis.

　　Results: Serum myocardial enzyme CK, LDH, myocardial cell apoptosis index and MDA in dry heat control group were significantly higher than that of normal temperature control group (P<0.01), curcumin pretreatment group CK, LDH, myocardial cell apoptosis index, MDA decreased with the increase of drug concentration, and the difference between curcumin pretreatment groups was significant (P<0.01); the change trend of SOD was opposite to MDA. Correlation analysis suggested that cardiomyocyte apoptosis and rat serum myocardial enzymes There is a positive correlation between CK and LDH (the correlation coefficient is r=0.30 r=0.30 P<0.05); there is a positive correlation between cardiomyocyte apoptosis and MDA (the correlation coefficient is r= 0.916, P<0.01); There is a negative correlation between cardiomyocyte apoptosis and SOD (the coefficient is r=-0.86 P<0.01).

　　Conclusion: Curcumin pretreatment has a protective effect on myocardial injury in rats with heat stroke in dry heat environment. Curcumin may play a protective effect on myocardial injury by resisting oxidative stress and reducing cell apoptosis.