动物造模 z-bio 2021-09-23 357

　　Objective: To study the distribution and expression of lysozyme 1 gene in mouse hatching blastocysts and dormant embryos before and after superovulation, and to explore the new regulatory mechanism during animal embryo implantation.

　　Methods: Normally hatched blastocysts and superovulated blastocysts obtained from 5d gestational ICR mice, and dormant embryos and superovulated dormant embryos were obtained on the 8th day of pregnancy using a mouse delayed implantation model. LYZ1 protein was detected by immunofluorescence and WesternBlot methods Distribution and differential expression changes in the four groups of embryos.

　　Results: LYZ1 was expressed in mouse hatching blastocysts and dormant embryos before and after superovulation, and was mainly concentrated in the inner cell mass, and was rarely distributed in trophoblast cells and cytoplasm; it was compared with mice that did not undergo superovulation. In contrast, the expression of LYZ1 protein was significantly up-regulated in mouse embryos after superovulation. Compared with mice without a dormant model, the expression of LYZ1 protein in dormant model mouse embryos was significantly up-regulated.

　　Conclusion: LYZ1 protein is expressed in the inner cell mass of the blastocyst, and may be involved in regulating the development of the inner cell mass of the embryo; the high expression of LYZ1 protein in superovulation-dormant embryos indicates that LYZ1 protein is under the dual influence of dormancy and superovulation. Will be adjusted upwards because of resistance to unfavorable environments.