动物造模,慢性萎缩性胃炎 z-bio 2021-09-09 461

　　Objective: To investigate the effect of Dendrobium officinale polysaccharide on chronic atrophic gastritis induced by nitrosoguanidine in rats and its molecular mechanism.

　　Methods: 120 rats were randomly divided into normal group, model group, Yangweishu group, Dendrobium officinale polysaccharide high, medium, and low dose group, 20 per group. Model group, Yangweishu group, Dendrobium officinale polysaccharide high, medium ､The low-dose group was given 167μg/mL MNNG drinking solution, and the normal group was free to drink distilled water for a total of 8 weeks. After the model was successfully established, the high-dose Dendrobium officinale polysaccharide group was given a daily dose of 1.34g/kg of Dendrobium officinale polysaccharide extract crude drug ; Medium-dose group 0.67g/kg; low-dose group 0.335g/kg. Yangweishu group was given Yangweishu granules 4.0g/kg daily by gavage. The model group was given normal saline gavage, and the normal group was given no intervention. Give After 12 weeks of treatment, the gastric tissue was removed for histopathology, gastrin (Gas), interleukin-6 (IL-6), proliferating Cell Nuclear Antigen (PCNA), tumor necrosis factor-α (tumornecrosis factor) α,TNF-α mRNA), Janus kinase 2 (JAK2), activator-3/tyrosine phosphorylation 3 (p-STAT3/STAT3) detection. Results After the 12th week of treatment, the normal group had high polysaccharides from Dendrobium candidum The body weight of the medium and low dose group and the Yangweishu group was higher than that of the model group (P<0.05). The weight of the Dendrobium officinale polysaccharide high and medium dose group was higher than that of the Yangweishu group (P<0.05); ②Inflammation grade of the model group Higher than the normal group (P<0.01), while the number of glands, thickness of glands, number of principal cells, and number of parietal cells are lower than the model group (P<0.05). Dendrobium officinale polysaccharide high, medium, and low dose group, nourishing the stomach The inflammation grade, number of glands, thickness of glands, number of principal cells, and number of parietal cells in the group were recovered compared with the model group (P<0.05). Compared with the Yangweishu group, the main cells of the Dendrobium officinale polysaccharide high and medium dose group Increase in number (P<0.05); the number of parietal cells in the high-dose Dendrobium officinale polysaccharide group increased (P<0.05); ③In the normal group, the high, medium, and low-dose Dendrobium officinale polysaccharide group, the serum GAS content of the Yangweishu group was higher than Model group (P<0.05). The normal group, Dendrobium officinale polysaccharide high-dose group had lower serum IL-6 levels than the model group (P<0.05); ④The normal group, Dendrobium officinale polysaccharide high-dose and medium-dose group PCNA and TNF- αmRNA was lower than the model group (P<0.05); ⑤In the normal group, the protein expression of Dendrobium officinale polysaccharide high and medium dose group JAK2, p-STAT3/STAT3 was lower than that of the model group (P<0.05). Conclusion Dendrobium officinale polysaccharide can affect CAG Rats have good protective and reversal effects, can effectively restore weight and relieve gastric mucosal atrophy. The mechanism of action may be to improve the pathological state of gastric mucosa by inhibiting the activation of JAK2/p-STAT3 signaling pathway.