动物造模 z-bio 2021-09-14 199

　　Objective To study the radioprotective effect of diallyl disulfide (DADS) on mouse spermatogenic cells.

　　Methods 4 Gy dose X-rays were used to establish an animal radiation injury model. The changes in testicular tissue, sperm motility and sperm deformity rate of irradiated mice were observed, and the protein carbonyl content, malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-hydroxydeoxyguanosine) were detected. OHdG) content, assess the degree of radiation damage of spermatogenic cells and the protective effect of DADS on spermatogenic cells. Measure testicular tissue antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione Peptide peroxidase (GSH-Px) activity and glutathione (GSH) content, western blot was used to detect the expression of Nrf2 signal protein, and the mechanism of radiation protection of DADS was explored.

　　Results Compared with the irradiation group alone, the DADS pretreatment group had less testicular tissue damage, significantly improved sperm motility (P<0.05), decreased sperm deformity rate (P<0.05), and significantly decreased protein carbonyl content, MDA and 8-OHdG levels (P<0.05). SOD, CAT, GSH-Px and GSH and other antioxidant indicators are significantly improved (P<0.05), and the expression of Nrf2 is significantly enhanced.

　　Conclusion DADS has a certain protective effect against acute radiation damage to spermatogenic cells of male mice through its antioxidant capacity.