动物造模,基因敲除小鼠,黑色素瘤 z-bio 2021-09-14 198

　　Objective: To investigate the effect of atrial natriuretic peptide on the growth of melanoma.

　　Methods: The melanoma B16F10 cells cultured in vitro were respectively inoculated subcutaneously in ANP knockout mice and C57BL/6J mice to establish a melanoma transplanted tumor model. From the 7th day, the long and short diameters of the tumor were measured, and the tumor's size was calculated. Volume; on the 12th day after tumor inoculation, the mice were sacrificed by cervical dislocation, the transplanted tumor was isolated and weighed. After the tumor tissue was embedded in paraffin, the tumor cell proliferation and microvessel density were detected by immunohistochemistry.

　　Results: The tumor volume of ANP knockout mice was significantly smaller than that of C57BL/6J mice, and the tumor weight was also significantly lighter. The number of cell proliferation and microvessel density in tumor tissues of ANP knockout mice were also significantly less than that of C57BL/6J mice.

　　Conclusion: ANP deletion may significantly inhibit the growth of melanoma by inhibiting tumor cell proliferation and microvessel formation.