动物造模 z-bio 2022-02-06 471

　　Purpose: By transplanting the feces of pigs with different feed utilization efficiency into pseudo-sterile mice, to explore its influence on the growth performance of recipient mice, and to initially reveal its mechanism.

　　Method: 36 pigs of 28kg Du×Long×Large barrows were raised in a single cage for 42 days (free intake) and their feed conversion efficiency was measured. At the end of the experiment, 36 pigs were classified as high, medium, and feed conversion efficiency. In the low 3 group, fresh feces from the three groups of pigs were collected and transplanted into mice treated with broad-spectrum antibiotics by gavage to monitor the growth performance of the mice after fecal transplantation. The total feces collection method was used to determine the high feed utilization efficiency. The nutrient digestibility of the middle and low groups of pigs was analyzed and compared by 16S rRNA V3-4 variable region amplification and sequencing to analyze and compare the fecal microbial composition of pigs and mice.

　　Results: After transplanting the feces of pigs with different feed utilization efficiency to pseudo-sterile mice treated with broad-spectrum antibiotics, the mice reproduced the growth performance phenotype of pigs; in terms of the composition and structure of intestinal microbes, pigs with high feed conversion efficiency The recipient mice with high growth performance have relatively high species abundance and microbial diversity of intestinal microbes; the total digestibility of pigs with high feed utilization efficiency (P=0.01) is significantly increased, and the abundance of Brevibacterium aerogenes is significant After fecal transplantation, the abundance of enterococcus and Akkermansia in recipient mice was also significantly higher than that of recipient mice with low feed utilization efficiency, indicating that gut microbes play an important role in the efficient use of energy. effect.

　　Conclusion: The transplantation of pig feces with different feed utilization efficiency can change the species richness, microbial diversity and growth performance of the intestinal microbes of pseudo-sterile mice. The advantage of high feed utilization efficiency pigs lies in higher energy digestion and utilization efficiency. The abundance of microorganisms related to energy efficient utilization in the intestine is relatively high.