动物造模 z-bio 2021-09-24 411

　　Objective: To explore a fast, convenient, and efficient new method for extracting rat dorsal root ganglia---searching and extracting DRG against the direction of nerve walking.

　　Method: The traditional method and the new method of opening the intervertebral foramen were used to extract the DRG, and the time consumed by the two methods of extraction and the number of complete DRGs were compared.

　　Results: Using traditional methods, the number of complete dorsal root ganglia from L3 to 5 segments per rat was (3.08 ± 1.31), and the average time per DRG was (5.58 ± 1.21) minutes. ; Using the new method, the number of complete dorsal root ganglia from L3 to 5 segments per rat is (4.29 ± 1.08), and the average time per DRG is (1.69 ± 0.91) minutes. Compared with the traditional method, the difference is significant (P<0.05).

　　Conclusion: The new method of searching and extracting DRG against the direction of nerve walking can more efficiently extract the complete rat dorsal root ganglion, which provides a more reliable method for the culture and morphological study of dorsal root ganglion cells in the later stage of the experiment. Organize materials.