动物造模 z-bio 2021-09-14 557

　　Objective: To explore the establishment of a model of emotional-cognitive behavior damage in mice caused by high-frequency electromagnetic radiation, and to provide an experimental basis for the research on the mechanism and protection of radiation damage.

　　Methods: The high-frequency electromagnetic reverberation chamber can produce continuous and uniform electromagnetic radiation waves. The mice were exposed to the high-frequency electromagnetic radiation box for 1d, 3d and 7d, and a series of behavioral tests were performed. Behaviors, depression-like behaviors, and changes in cognitive function.

　　Results: High-frequency electromagnetic radiation (frequency 3GHz, SAR value 4W/kg) was exposed for 4 hours a day and continuously exposed for 7 days. It did not affect the autonomous activities of the mice, but it could cause the number of times the mice in the radiation group entered the central area of the opening (P<0) .05) and time (P <0.05) were significantly reduced; compared with the unexposed group, mice in the radiation group significantly reduced the number of times (P <0.05) and time percentage ( P <0.01); at the same time, exposure to electromagnetic radiation at this dose also significantly increased the immobility time of mice in forced swimming and tail suspension experiments (P <0.01, P <0.05); and compared with the radiation exposure group The mice in the unexposed group significantly reduced the discrimination index of new objects in the new object recognition experiment (P <0.001).

　　Conclusion: Continuous high-frequency electromagnetic radiation (frequency of 3 GHz, SAR value of 4 W/kg) can time-dependently cause anxiety-depression-like behaviors and cognitive impairment in mice, which provides an experiment for related prevention and treatment technology research in accordance with.