动物造模,造血干细胞 z-bio 2021-09-15 776

　　Objective: To detect the effects of different administration methods on the function of hematopoietic stem cells in mice.

　　Method: C57 mice were given busulfan intraperitoneally, divided into high-dose group (40 mg/kg) and low-dose group (20 mg/kg), the low-dose group was given once, and the high-dose group was given for two days Administration, 20 mg/kg per day. 15 d and 30 d after administration, the mouse peripheral blood and bone marrow cell counts, hematopoietic stem cells, hematopoietic progenitor cells and long-term hematopoietic stem cell ratios were measured, respectively, and the proliferation ability of mouse hematopoietic progenitor cells was evaluated by CFU-GM experiment. Flow sorting was long-term Hematopoietic stem cells are cultivated in vitro, and the function of hematopoietic stem cells is tested by single cell colony experiment.

　　Result: Both the high-dose group and the low-dose group of busulfan can reduce the number of white blood cells and platelets in the peripheral blood of C57 mice, reduce the proportion of hematopoietic stem progenitor cells and long-term hematopoietic stem cells, and reduce the ability of CFU-GM and single cell colony formation. The injury effect of the high-dose group was worse than that of the low-dose group. There was no significant difference between the weight of mice and the control group.

　　Conclusion: The high-dose group of busulfan can induce hematopoietic stem cell damage after 15 days, which provides a model and basis for the discussion of the mechanism of hematopoietic stem cell damage and the study of damage protection.