动物造模 z-bio 2021-09-15 713

　　Purpose: To observe the changes in dopamine (DA) content in the lateral geniculate body in guinea pig stroboscopic-induced myopia and form-deprivation myopia models, and stop the comparative analysis, and discuss the comparison of the central pathogenesis of different myopia models.

　　Methods: 24 ordinary 2 weeks old guinea pigs were randomly divided into 3 groups (n = 8): stroboscopic light (FLM) group? Form deprivation (FDM) group? Control group, each group was kept for 8 weeks? Before and after modeling Measure the refractive power and axial length of the right eye of each group of guinea pigs. After the 8-week experiment, high-performance liquid chromatography electrochemical detection (HPLC?ECD) was used to quantify DA in the left lateral geniculate body.

　　Results: Before modeling, there was no significant difference in refractive power and axial length of each group (P>0.05). At the 8th week of modeling, compared with the control group, the change in refractive power of the right eye in the FLM and FDM groups (P<0.001) ? The changes in the axial length of the eye (P <0.05) are="" suggesting="" the="" success="" of="" myopia="" hplc-ecd="" da="" flm="">control group> FDM group; control group is (37.04 ±1.18)pg / μL; FDM group is (24.27 ± 3.46) pg/ μL, compared with the control group, the difference is significant (P = 0.021); FLM group is (45.58 ± 1.98) pg/ μL, compared with the control group, the difference is significant Sex (P=0.01)?

　　Conclusion: The DA content in the lateral geniculate body of the stroboscopic induced myopia model increased, while the DA content in the form-deprived myopia model decreased, indicating that the expression of DA in the lateral geniculate body of the two experimental myopia is not different, and the two myopia models The onset mechanism may be different?