动物造模 z-bio 2022-02-01 488

　　OBJECTIVE: To study the induced differential expression of integrin αvβ3 gene in bovine uterine epithelial cells cultured in vitro, in order to provide a reference for exploring the marker protein of bovine uterine receptivity.

　　Method: Use RT-PCR to analyze the changes in the induced expression of integrin αvβ3 in bovine endometrial epithelial cells by the synergistic effect of different concentrations of estrogen, progesterone, and estrogen and progesterone.

　　Results: When progesterone 10-7 mg/mL was added alone, the expression of integrin αvβ3 was the highest, and the expression of αv and β3 in the 10 -7 mg/mL group were significantly higher than those in the control group (P<0.05)? Add alone In the estrogen 10-10 mg/mL group, the expression of αv was the highest, while the expression of β3 was the lowest. In the co-addition of estrogen and progesterone, the expression of integrin αvβ3 was higher than that of the control group, and the expression of αv was significant. Higher than the control group (P<0.05); 3="" p="">0.05)?

　　Conclusion: When progesterone is added alone and estrogen is used in combination, both can promote the increase of integrin αvβ3 mRNA expression in bovine endometrial epithelial cells; adding estrogen alone has the opposite effect? Therefore, integrin αvβ3 can be used as a potential reference marker gene for uterine receptivity in bovine endometrial epithelium during the "planting window" period?